Receptor Binding Techniques

Beschreibung

In Receptor Binding Techniques, detailed experimental protocols devised by expert practitioners demonstrate the power of radioligand binding for studying a wide variety of receptors. The methods can be applied to G protein-coupled receptors, receptors with integral ion channels, and sites other than receptors, such as guanine nucleotide binding proteins, enzymes, transporters, and mRNA. The individual protocols encompass many different preparations, ranging from solubilized receptors, through membrane preparations, intact cells, and autoradiography in tissue slices, to PET and SPECT analysis of binding in living human brain. Along with the detailed, easily reproducible instructions, the protocols also take into account the problems inherent in the basic binding technique, such as common experimental artifacts and the definition of receptor sites, as well as problems associated with the use of particular ligands and receptor preparations.

Receptor Binding Techniques shows clearly how these techniques can be adapted to many different applications and fully utilized in drug discovery programs. It will be of high value not only to researchers new to the field of receptor binding, but also to more experienced workers, serving as an authoritative bench manual replete with optimalized practical methods and protocols.

Pharmacologists, neuroscientists, cell biologists, molecular biologists, and biochemists


Radioligand binding is essentially a very simple technique, applicable to a wide range of preparations. It can of course be used to study many diff- ent types of receptors, but can also be adapted to investigate a variety of nonreceptor proteins, such as enzymes and uptake sites. The basic technique allows the affinity of drugs for the receptor of interest to be determined very easily, and the speed and precision of binding have led to its widespread use as a primary screen in drug discovery programs. It also allows receptor n- ber to be measured and the kinetics of the receptor-ligand interaction to be determined, parameters not readily accessible by any other means. Receptor distribution can be studied in great detail using the technique of autoradiog- phy; radioligand binding provides the most sensitive technique for dissecting allosteric interactions between ligands and receptors, and even between rec- tors and effector molecules, such as G proteins. Moreover, by allowing rec- tors to be identified in the absence of any measurable response, binding is a prerequisite for receptor solubilization and purification studies. It is even p- sible to measure ligand receptor interactions in vivo, using positron emission tomography and other powerful techniques. provides detailed experimental protocols for the measurement of binding to various receptor and nonreceptor sites in a range of preparations, including solubilized receptors, membrane preparations, whole cells, autoradiography, and PET and SPECT analysis in living human subjects.

Autor*in

Mary Keen

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"Research scientists who study or wish to study drug/hormone actions would benefit from this book. It is applicable for students, instructors, and advanced scientists. The editor has successfully compiled this book for the intended readers. . .I find this book a very useful and practical addition to the field of receptor pharmacology. . .This book should be open and on the bench of every student of receptor pharmacology.-Doody's Health Sciences Book Review Journal

"...step-by-step experimental protocols demonstrate the power of the radioligand binding...an extremely useful book."-Cellular and Molecular Biology

"...found the book easy to read and full of useful information. Having been involved in receptorology during the past 16 years, I found the content of this book comprehensive and up to date and as such the book will be useful for both the specialist as well as one just beginning in the area."-Cell Biology International

"...does much to dispel the mysteries surrounding the technique and provides a good many solutions to practical problems...The book is written in a uniformly accessible style and there is plenty of benefit to be derived from it for both novices and more experiences scientists wanting to learn a new technique."-Microbiology Today
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