Green Fluorescent Protein
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Beschreibung
Green fluorescent protein (GFP), a critically important marker protein, allows direct visualization in live samples without the aid of additional sample preparation. In Green Fluorescent Protein: Applications and Protocols, Barry W. Hicks and a team of well-practiced experimentalists demonstrate the power and the versatility of this marker with a cutting-edge collection of readily reproducible GFP techniques. These include methods for detecting and imaging GFP (FRET, BRET, and whole-body imaging), for using GFP to create and select for transgenic organisms, for its use as a biosensor, and for studying viruses. Each method is described in step-by-step detail to ensure robust results and can be used in applications ranging from agriculture and botany to human gene therapy. Extensive notes review the materials required, explain the scientific basis of each step and possible sources of error, and provide tips for overcoming problems.
Highly practical and state-of-the-art, Green Fluorescent Protein: Applications and Protocols makes available to all biological and biomedical experimentalists a full array of the powerful new GFP techniques.
Could there be a better time to be a life scientist? In the past two decades, a host of new techniques have been added to the tool chests of biochemists and molecular biologists. A wonderful benefit of the basic scientific research that fueled the advances in these fields is the wide variety of direct applications in agriculture and medicine. Even with all of these advances, and with the accompanying explosion in computer and information technology, it is clear that the depth of our ignorance vastly exceeds the breadth of our knowledge about complex organisms at the molecular level. Any new techniques or materials that allow us to extend our research-based knowledge should be w- comed and utilized to their fullest potentials. With the cloning of the green fluorescent protein (GFP) from Aequorea victoria in 1992, another valuable tool was added to the arsenal. In Green Fluorescent Proteins: Applications and Protocols examples of how GFP can be utilized in a variety of fields are presented. Although the text has chapters that emphasize different areas of s- cialization, it is not meant to send molecular biologists to one section, botanists to another, and clinicians to still another. Perhaps the most valuable exchange for people in any discipline will come from seeing how others have been able to apply GFP in fields outside of their immediate areas of expertise. GFP from Aequorea victoria is a fluorescent marker protein, and there are certainly other useful fluorophore markers.
Includes supplementary material: sn.pub/extras
Autor*in
Barry W. Hicks
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"The breadth of applications for green fluorescent protein will make this book useful for many laboratories and may provide new approaches for others not previously familiar with gfp as a tool. Gfp has provided a wide ranging and valuable tool for many investigators. This is a very useful set of protocols, even for those who don't think they have an application for the technique. It is especially valuable to have the diverse methods compiled in a single book. Most will find something of value." -Doody's Health Sciences Book Review Journal
"...this book truly is a collection of "Applications and protocols" and such is the degree of detail in the methods described that it should perhaps have been sub-titled "A Laboratory Manual." -The Analyst
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