G-Quadruplex DNA
Methods and Protocols
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Beschreibung
Recent work has revealed that stabilizing G-quadruplexes in telomeric DNA inhibits telomerase activity, providing impetus for the development of G-quartet-interacting drugs, while G-quartet-containing oligonucleotides have been recognized as a potent class of aptamers effective against STAT3 and other transcription factors implicated in oncogenesis, proving these guanine-quartets to be a vital and rich area for future study. In G-Quadruplex DNA: Methods and Protocols, experts in the field present a collection of detailed techniques for studying G-quartet formation, dynamics, and molecular recognition. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, G-Quadruplex DNA: Methods and Protocols promises to be a useful resource for those familiar with G-quartets as well as an easy entry point for those researchers from diverse fields who are just developing an interest in the exciting implications of G-quadruplex DNA.
Recent work has revealed that stabilizing G-quadruplexes in telomeric DNA inhibits telomerase activity, providing impetus for the development of G-quartet-interacting drugs, while G-quartet-containing oligonucleotides have been recognized as a potent class of aptamers effective against STAT3 and other transcription factors implicated in oncogenesis, proving these guanine-quartets to be a vital and rich area for future study. In "G-Quadruplex DNA: Methods and Protocols", experts in the field present a collection of detailed techniques for studying G-quartet formation, dynamics, and molecular recognition. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, "G-Quadruplex DNA: Methods and Protocols "promises to be a useful resource for those familiar with G-quartets as well as an easy entry point for those researchers from diverse fields who are just developing an interest in the exciting implications of G-quadruplex DNA.
Includes coverage of a wide range of methodologies to study G-quadruplex structures Provides detailed protocols which ensure successful re-recreation of experimental procedures Supplies step-by-step instructions in order to enable researchers to set up their own high throughput screens for G-quadruplex ligands Explores computational approaches for the detection of G-quadruplex-forming structures Examines state of the art physical, chemical, and biological methods Contains tricks of the trade and valuable troubleshooting advice Includes supplementary material: sn.pub/extras
Autor*in
Peter Baumann
Themen in »G-Quadruplex DNA«
DNA Drug development G-quartet formation and dynamics Guanine bases Molecular recognition Oligonucleotides Telomerase activity microscopy molecular biology transcription
Stimmen zu »G-Quadruplex DNA«
From the reviews:
“The book G-Quadruplex DNA … provides an excellent collection of important methods for the study of the DNA G-quadruplex structures. … I definitely recommend this book, which can be considered an outstanding source of information that addresses several problems … in the investigation of G-quadruplex structures. The book is appropriate for everyone working in the area of G-quadruplex research, including biologists, biochemists and chemists since it is a worthy guide for both beginners and experienced scientists interested in studying this increasingly attractive field.” (Aldo Galeone, ChemBioChem, Issue 11, September, 2010)()