Kidney Research
Experimental Protocols
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Beschreibung
As the expense of treating a growing number of end-stage kidney disease patients increases, greater attention has been paid to prevention and early treatment. The study of renal disease, however, suffers due to the complex nature of renal anatomy and physiology and the plethora of different cell types found in the kidney. In Kidney Research: Experimental Protocols, top experts in the field seek to aid researchers by providing a number of specialized techniques developed to examine this intricate system. Through both well-established and novel methods, this volume explores the preparation and culture of the main cell types used to study renal disease mechanisms, the common animal models used to mimic the various forms of human renal disease, and specific applications and techniques used in vivo and in vitro. Written in the highly successful Methods in Molecular Biology™ series format, the chapters contain step-by-step, readily reproducible laboratory protocols, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.
Essential and cutting-edge, Kidney Research: Experimental Protocols delivers invaluable guidance to new and experienced laboratory researchers delving into the sophisticated study of the kidney.
As the expense of treating a growing number of end-stage kidney disease patients increases, greater attention has been paid to prevention and early treatment. The study of renal disease, however, suffers due to the complex nature of renal anatomy and physiology and the plethora of different cell types found in the kidney. In Kidney Research: Experimental Protocols, top experts in the field seek to aid researchers by providing a number of specialized techniques developed to examine this intricate system. Through both well-established and novel methods, this volume explores the preparation and culture of the main cell types used to study renal disease mechanisms, the common animal models used to mimic the various forms of human renal disease, and specific applications and techniques used in vivo and in vitro. Written in the highly successful Methods in Molecular Biology™ series format, the chapters contain step-by-step, readily reproducible laboratory protocols, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.
Essential and cutting-edge, Kidney Research: Experimental Protocols delivers invaluable guidance to new and experienced laboratory researchers delving into the sophisticated study of the kidney.
Includes step-by-step instructions providing a level of detail rarely found in journal articles Provides experimental techniques for examining the mechanisms and pathology of kidney disease Details protocols for isolating and culturing the most common renal cell types Involves an in-depth review of the various animal models of renal disease Emphasizes techniques for mouse models Contains detailed methods for both gene and protein analysis in the kidney Examines methodology for in vivo gene transfer
Autor*in
Tim D. Hewitson
Themen in »Kidney Research«
Cell isolation Cell propagation In vivo and in vitro Laboratory Nephrology Renal cells Renal disease Termination electron microscopy gene expression genes molecular biology signal transduction tissue
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From the reviews:
“This book details research methodologies especially relevant for nephrological studies. … Researchers in the field of nephrology are the intended audience. The book covers research methodologies used in renal studies (as well as in other studies), including proteomics, micro-RNA work, and other cutting-edge approaches. … This is a unique book that has no competitors.” (Jens Goebel, Doody’s Book Reviews, December, 2011)()