Electron Microscopy

Beschreibung

In this revised and expanded second edition, Electron Microscopy: Methods and Protocols presents the newest technology in electron microscopy, while maintaining the practicality and accessibility of the acclaimed first edition. Like the first edition, this volume provides clear, concise instructions on processing biological specimens and includes discussion on the underlying principles of the majority of the processes presented. Each protocol is presented by experts with years of first-hand experience and in-depth knowledge of the techniques they describe. The Notes section accompanying each protocol will prove invaluable for adapting and troubleshooting protocols.

Electron Microscopy: Methods and Protocols, Second Edition, comprises two major areas of electron microscopy-transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The TEM area covers several key techniques, including: conventional specimen preparation methods for cultured cells and biomedical and plant tissues; cryospecimen preparation by high-pressure freezing and cryoultramicrotomy negative staining and immunogold labeling techniques; and TEM crystallography and cryo-TEM tomography. The SEM area similarly attends to conventional-, variable pressure-, environmental-, and cryoscanning microscopy techniques, as well as the application of X-ray microanalysis. Protocols for the application of X-ray microanalysis to SEM and mass spectrometry conclude the volume.

Essential electron microscopy techniques for TEM and SEM

Cutting-edge cryo-EM and 3D TEM techniques

Quantitative aspects of immunogold labeling in resin embedded samples

Examines the application of electron energy-loss spectroscopy (EELS) to biomedical tissue

Notes section enables efficient adaptation and troubleshooting of protocols

Autor*in

John Kuo

Themen in »Electron Microscopy«

DNA biological materials electron microscopy hybridization membrane proteins molecular biology scanning electron microscope transmission electron microscopy

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"This is an excellent book, with well-written chapters.  The protocols are easy to follow and well organized.  This book is highly recommended for beginners to advanced level microscopists." -Micron
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