Human Embryonic Stem Cell Protocols
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Beschreibung
Despite political and ethical controversies surrounding the study of human embryonic stem (hES) cells, interest remains high in understanding the regulatory mechanisms of stem cell self-renewal, their differentiation along various lineages, and their potential use in regenerative medicine. In Human Embryonic Stem Cell Protocols, internationally respected researchers describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells. This diverse collection of readily reproducible methods has been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion CD provides color versions of all illustrations in the book.
Comprehensive and cutting-edge, Human Embryonic Stem Cell Protocols offers both novice and expert researchers powerful tools essential to understanding the maintenance and differentiation of human embryonic stem cells, as well as their applications in regenerative medicine today.
A comprehensive collection of diverse techniques for the molecular and cellular manipulation of human embryonic stem (hES) cells. These readily reproducible methods have been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. A companion CD provides color versions of all illustrations in the book. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Human Embryonic Stem Cell Protocols contributes to progress in the field by investigating human embryonic stem cells (hES) and increasing our understanding of the regulation of their self-renewal capacity, commitment and differentiation along various lineages, and their potential utility in regenerative medicine applications. These hard-won protocols, all generously shared by experts in this field, support the continued isolation of additional hES lines as well as the development of improved culture conditions countering the view that hES cells are difficult to maintain and use.
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From the reviews:
"The book presents 22 chapters from an international group of experts in the field … . Human Embryonic Stem Cell Protocols is designed to provide a collection of the essential methods in hESC culture and maintenance … . a comprehensive set of valuable protocols from leading research labs in the world. … This book is a valuable resource for hESC investigators who hope to expand their understanding of the manipulation of hESCs." (Elayne Chan and George Q. Daley, Bone Marrow Transplantation, May, 2006)
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