Zinc Finger Proteins
Methods and Protocols
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Beschreibung
This detailed volume explores the notable progress in the field of zinc finger proteins (ZFP) study through widely used methods and protocols involving their biological functions and applications. Beginning with a section on the basic biology of ZFPs and design and applications of custom ZFPs, the book continues by covering methods for the evaluation and prevention of ZFN-mediated cytotoxicity as well as a collection of the representative methods of ZFN delivery. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Zinc Finger Proteins: Methods and Protocols aims to prompt both the understanding of ZFP biology and the development of next-generation ZFP therapeutics.
This detailed volume explores the notable progress in the field of zinc finger proteins (ZFP) study through widely used methods and protocols involving their biological functions and applications. Beginning with a section on the basic biology of ZFPs and design and applications of custom ZFPs, the book continues by covering methods for the evaluation and prevention of ZFN-mediated cytotoxicity as well as a collection of the representative methods of ZFN delivery. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Zinc Finger Proteins: Methods and Protocols aims to prompt both the understanding of ZFP biology and the development of next-generation ZFP therapeutics.
Includes cutting-edge methods and protocols for the study of zinc finger proteins Provides step-by-step detail essential for reproducible results Contains notes and implementation advice from the experts
Autor*in
Jia Liu
Themen in »Zinc Finger Proteins«
ZFP domains Genome sequence targeting Gene expression Genomic engineering Delivery methods ZFN-induced genotoxicity Next-generation ZFP therapeutics