Non-Viral Gene Delivery Vectors
Methods and Protocols
Buch in deiner Nähe kaufen
Beschreibung
This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field of
non-viral gene delivery vectors. The chapters in this book are organized into
three major parts: Part I is a section on conventional bolus gene delivery
vectors that introduces typical transfection approaches relying
on the addition of transfectants to the cell culture medium where the cells are
grown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers or
stimuli-responsive polymers that change according to the environment they are
in and delivered by dripping into cells; Part III discusses examples of
substrate-mediated gene delivery—also termed reverse
transfection—and the immobilization of a gene delivery vector onto a surface as
opposed to more typical bolus delivery from the medium. Written in the highly
successful Methods in Molecular Biology series format, chapters include
introductions to their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols, and tips on
troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Non-Viral Gene Delivery Vectors: Methods and
Protocols is written for experimentalists, and is an essential part of many
laboratory bookshelves. This book will help novice and professionals alike
succeed in their research in this field.
This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field of non-viral gene delivery vectors. The chapters in this book are organized into three major parts: Part I is a section on conventional bolus gene delivery vectors that introduces typical transfection approaches relying on the addition of transfectants to the cell culture medium where the cells are grown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers or stimuli-responsive polymers that change according to the environment they are in and delivered by dripping into cells; Part III discusses examples of substrate-mediated gene delivery—also termed reverse transfection—and the immobilization of a gene delivery vector onto a surface as opposed to more typical bolus delivery from the medium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Non-Viral Gene Delivery Vectors: Methods and Protocols is written for experimentalists, and is an essential part of many laboratory bookshelves. This book will help novice and professionals alike succeed in their research in this field.
Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras
Autor*in
Gabriele Candiani
Themen in »Non-Viral Gene Delivery Vectors«
conventional bolus gene delivery vectors cell culture medium stimuli-responsive bolus transfectants polymers reverse transfection solid-phase delivery cationic lipid-based lipoplexes zwitterionic lipid-based lipoplexes polymer-strenthened lipid-coated