Histone Deacetylases
Methods and Protocols
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Beschreibung
This volume provides different methodologies for all classes of histone deacetylases, which includes detailed procedures on Class I and II histone deacetylase inhibitors, SIRT inhibitors, and bromodomain inhibitors. Histone Deacetylases: Methods and Protocols is divided into four sections: Sections A and B describe methodologies used to detect the activity, function, or chromatin location of HDACs 1 through 11, with Section A discussing Class I and Section B discussing class II histone deacetylases; Section C focuses on the methodologies for cloning and characterizing the acetylation of SIRTs 1 through 7; and Section D describes methods related to histone deacetylase inhibitors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Histone Deacetylases: Methods and Protocols, is a valuable resource for investigators working on epigenetics, molecular biology, and genetics.
This volume provides different methodologies for all classes of histone deacetylases, which includes detailed procedures on Class I and II histone deacetylase inhibitors, SIRT inhibitors, and bromodomain inhibitors. Histone Deacetylases: Methods and Protocols is divided into four sections: Sections A and B describe methodologies used to detect the activity, function, or chromatin location of HDACs 1 through 11, with Section A discussing Class I and Section B discussing class II histone deacetylases; Section C focuses on the methodologies for cloning and characterizing the acetylation of SIRTs 1 through 7; and Section D describes methods related to histone deacetylase inhibitors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Histone Deacetylases: Methods and Protocols, is a valuable resource for investigators working on epigenetics, molecular biology, and genetics.
Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts
Autor*in
Sibaji Sarkar
Themen in »Histone Deacetylases«
epigenetics deacetylation of histones in the nucleus deacetylation of cytoplasmic signaling proteins sirtuins inhibitors (SIRTs) histone deacetylase (HDACs) inhibitors bromodomain inhibitors acetylation of SIRTs enzymes
Stimmen zu »Histone Deacetylases«
“This volume of Methods in Molecular Biology, Histone deacetylases, provides comprehensive overview of the methods and procedures for analysis of histone modifications, namely histone deacetylation. … Methodological tools required for more detailed determination of the molecular and biological roles of distinct HDACs and HDAC-containing complexes are summarized in this book, which will no doubt hasten these important mechanistic studies.” (Bozena Smolkova, Neoplasma, Vol. 64 (3), 2017)
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