mRNA Processing
Methods and Protocols
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Beschreibung
This detailed volume assembles a number of the most commonly used and state-of-the-art methodologies in the revolutionary field of mRNA processing in order to aid researchers in choosing the best method for their specific problems. Given that the vast majority of alternative mRNA processing events has yet to be explored, these techniques promise to breathe new life into the myriad new ideas currently underway. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and easy to use, mRNA Processing: Methods and Protocols seeks to aid scientists venturing into what’s certain to be another explosive period of growth in this dynamic field of study.
This detailed volume assembles a number of the most commonly used and state-of-the-art methodologies in the revolutionary field of mRNA processing in order to aid researchers in choosing the best method for their specific problems. Given that the vast majority of alternative mRNA processing events has yet to be explored, these techniques promise to breathe new life into the myriad new ideas currently underway. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and easy to use, mRNA Processing: Methods and Protocols seeks to aid scientists venturing into what’s certain to be another explosive period of growth in this dynamic field of study.
The chapter 'Robust, Cost-EffectiveProfiling of RNA Binding Protein Targets with Single-end Enhanced Crosslinking and Immunoprecipitation (seCLIP)' is published open access under a CC BY 4.0 license at link.springer.com.
Includes cutting-edge methods and protocols for the study of mRNA processing Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras
Autor*in
Yongsheng Shi
Themen in »mRNA Processing«
Metabolic labeling Bioinformatic tools Splicing patterns Alternative polyadenylation Circular RNAs