DNA Electrophoresis Protocols for Forensic Genetics
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Beschreibung
Forensic DNA profiling procedures are mainly based on high resolution and high throughput capillary electrophoresis separation and detection systems of PCR amplicons obtained from DNA genomic markers with different inheritance patterns. In DNA Electrophoresis Protocols for Forensic Genetics, expert researchers in the field detail many of the protocols and methods which are now commonly used to perform forensic DNA profiling. It includes protocols for profiling of autosomal STRs, Y-STRs, X-STRs, autosomal SNPs, INDELS, Y-SNPs, mtDNA-SNPs, and mtDNA hypervariable regions HV1 and HV2 . Protocols for molecular identification of non-human species and mRNA profiling for body fluid identification are also included. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Forensic DNA profiling procedures are mainly based on high resolution and high throughput capillary electrophoresis separation and detection systems of PCR amplicons obtained from DNA genomic markers with different inheritance patterns. In DNA Electrophoresis Protocols for Forensic Genetics, expert researchers in the field detail many of the protocols and methods which are now commonly used to perform forensic DNA profiling. It includes protocols for profiling of autosomal STRs, Y-STRs, X-STRs, autosomal SNPs, INDELS, Y-SNPs, mtDNA-SNPs, and mtDNA hypervariable regions HV1 and HV2 . Protocols for molecular identification of non-human species and mRNA profiling for body fluid identification are also included. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Offers comprehensive coverage of the most modern current electrophoretic protocols and interpretation guidelines used in forensic genetics Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras
Autor*in
Antonio Alonso
Themen in »DNA Electrophoresis Protocols for Forensic Genetics«
DNA STR markers autosomal STRs capillary electrophoresis forensic botany forensic genetics forensic veterinary microbial forensic molecular identification sex chromosome markers Electrophoresis
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From the reviews:
“The purpose is to offer a comprehensive book on the most modern current electrophoretic protocols and interpretation guidelines used in forensic genetics for the analysis of amplified human DNA fragments and DNA sequencing. … researchers, forensic experts, and others interested in the field of DNA electrophoresis protocols will find this book of immense help. … This is a unique resource that students, researchers, and forensic experts will find both interesting and helpful.” (Omer Iqbal, Doody’s Book Reviews, May, 2012)()