Stem Cell Migration
Methods and Protocols
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Beschreibung
The migration of stem cells has been found to be critical during early development for the organization of the embryonic body as well as during adult life with tissue homeostasis and regeneration of organ function. Due to the low frequency of these cells in vivo, problems in identifying and prospectively purifying tissue specific stem cells near homogeneity, and, most importantly, a lack of adequate technologies and protocols to study stem cell migration in vivo, this vital research has been quite difficult until recently. In Stem Cell Migration: Methods and Protocols, experts in the field compile and highlight the standard and novel techniques that allow the studying of the migration of stem cells in one succinct manual. Including protocols on germ, neuronal, and hematopoietic stem cells, during development and adulthood with a clear emphasis on in vivo technologies, the volume also extends its coverage to in vitro approaches toward several developmentally-conserved signaling pathways. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and convenient, Stem Cell Migration: Methods and Protocols provides key, state-of the art information on experimental techniques for studying stem cell migration both at a cellular and molecular level in development, regeneration, and disease.
The migration of stem cells has been found to be critical during early development for the organization of the embryonic body as well as during adult life with tissue homeostasis and regeneration of organ function. Due to the low frequency of these cells in vivo, problems in identifying and prospectively purifying tissue specific stem cells near homogeneity, and, most importantly, a lack of adequate technologies and protocols to study stem cell migration in vivo, this vital research has been quite difficult until recently. In Stem Cell Migration: Methods and Protocols, experts in the field compile and highlight the standard and novel techniques that allow the studying of the migration of stem cells in one succinct manual. Including protocols on germ, neuronal, and hematopoietic stem cells, during development and adulthood with a clear emphasis on in vivo technologies, the volume also extends its coverage to in vitro approaches toward several developmentally-conserved signaling pathways. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and convenient, Stem Cell Migration: Methods and Protocols provides key, state-of the art information on experimental techniques for studying stem cell migration both at a cellular and molecular level in development, regeneration, and disease.
Provides a succinct manual to the cutting-edge techniques in stem cell migration Includes clear and practical step-by-step protocols, perfect for reproducible results Features tips and key implementation advice from the experts Includes supplementary material: sn.pub/extras
Autor*in
Marie-Dominique Filippi
Themen in »Stem Cell Migration«
Cancer stem cells Embryogenesis In vivo technologies Migration and disease Organ regeneration Signaling pathways Tissue homeostasis
Stimmen zu »Stem Cell Migration«
From the reviews:
“This well-illustrated book details the techniques essential for the understanding of the migration of hematopoietic stem cells, neural stem cells, and primordial germ cells. … The book is well illustrated with both color and black-and-white figures. This is a highly informative manual of novel techniques dealing with stem cell migration both at a cellular and molecular level in development, regeneration, and disease. Students, researchers and physician-scientists interested in the areas of stem cell migration will find this book useful.” (Omer Iqbal, Doody’s Book Reviews, October, 2011)()