Brain Development
Methods and Protocols
Buch in deiner Nähe kaufen
Beschreibung
Providing widely used techniques in genetic model systems and many complementing animal models, Brain Development: Methods and Protocols focuses its expert contributions on two key technical aspects of developmental neurobiology: detection of gene expression and functional characterization of developmental control genes. Covering animal models such as the fruit fly, zebra fish, chicken, and mouse, this detailed book views in situ hybridization, reporter gene expression, and immunohistochemical staining methods, as well as RNA interference, Morpholino, or transgenic techniques through the prism of these models. Written in the highly successful Methods in Molecular Biology series format, chapter include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and cutting-edge, Brain Development: Methods and Protocols aims to provide precise technical protocols but also allows for comparing a wide range of protocols in different tissues and species.
Providing widely used techniques in genetic model systems and many complementing animal models, Brain Development: Methods and Protocols focuses its expert contributions on two key technical aspects of developmental neurobiology: detection of gene expression and functional characterization of developmental control genes. Covering animal models such as the fruit fly, zebra fish, chicken, and mouse, this detailed book views in situ hybridization, reporter gene expression, and immunohistochemical staining methods, as well as RNA interference, Morpholino, or transgenic techniques through the prism of these models. Written in the highly successful Methods in Molecular Biology series format, chapter include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and cutting-edge, Brain Development: Methods and Protocols aims to provide precise technical protocols but also allows for comparing a wide range of protocols in different tissues and species.
Introduces cutting-edge techniques in developmental neurobiology through genetic model systems as well as some complimentary animal models Provides step-by-step detail essential for reproducible results Contains key notes and expert implementation advice
Autor*in
Simon G Sprecher
Themen in »Brain Development«
Animal models Developmental control genes Developmental neurobiology Drosophila Gene expression detection Genetic model systems Zebrafish