Light Microscopy

Beschreibung

Of all scientific instruments, probably none has had more applications in the life sciences than the light microscope. In Light Microscopy: Methods and Protocols, expert researchers explore the basics and the latest advances in microscope instrumentation, sample preparation, and imaging techniques, all of which have been producing fundamental insights into the functions of cells and tissues. Chapters cover a variety of bright field and fluorescence microscopy-based approaches that are central to the study of a range of biological questions, providing information on how to prepare cells and tissues for microscopic investigations, covering detailed staining procedures, and exploring methods to analyze images and interpret the results accurately. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Light Microscopy: Methods and Protocols is an essential handbook for all researchers who are exploring the intriguing microscopic world of the cell.
Of all scientific instruments, probably none has had more applications in the life sciences than the light microscope. In Light Microscopy: Methods and Protocols, expert researchers explore the basics and the latest advances in microscope instrumentation, sample preparation, and imaging techniques, all of which have been producing fundamental insights into the functions of cells and tissues. Chapters cover a variety of bright field and fluorescence microscopy-based approaches that are central to the study of a range of biological questions, providing information on how to prepare cells and tissues for microscopic investigations, covering detailed staining procedures, and exploring methods to analyze images and interpret the results accurately. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Light Microscopy: Methods and Protocols is an essential handbook for all researchers who are exploring the intriguing microscopic world of the cell.
Readily reproducible protocols for optimal histological observations under bright-field microscopy Provides full description of plastic resin embedding methods for plant and animal research Presents advanced methods to study leukocyte biology and inflammatory responses Imaging techniques applied to cytogenetic, cancer, neurobiology, ion mobilization, and aquatic ecology studies Includes immunocytochemistry methods and a comprehensive overview of fluorescence microscopy Includes supplementary material: sn.pub/extras

Autor*in

Hélio Chiarini-Garcia

Themen in »Light Microscopy«

Bright-field microscopy Microscope instrumentation Plastic resin embedding methods Sample preparation Staining procedures

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From the reviews:

“The book is superbly illustrated with the great majority of the figures in colours and is certainly a must not only for beginners … but I am pretty sure that even the practised colleagues in doing every day use of microscopic methods will find very useful tricks … . thanks to this well planned and clearly written book we are stimulated to imagine new applications.” (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)

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