Chromatin Protocols
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Beschreibung
Significant advancements have been made in the study of chromatin structure and function over the past fifty years but none as spectacular as those made in the last decade due to the development of novel techniques and the ability to sequence large stretches of DNA. In Chromatin Protocols, Second Edition, expert researchers delineate these cutting-edge techniques via step-by-step laboratory methods and protocols, which encompass a wide array of topics from the isolation of nucleosomes, assembly of nucleosomes and study of the basic chromatin structure to detailed analysis of histone modifications and chromatin function. Written in the highly successful Methods in Molecular Biology™ series style, chapters include brief introductions to the subjects, lists of the necessary materials and reagents, readily reproducible protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Chromatin Protocols, Second Edition is a valuable tool for scientists studying various aspects of chromatin function and an ideal guide to aid in the development of new techniques as well as new ideas in the field of chromatin biology.
Significant advancements have been made in the study of chromatin structure and function over the past fifty years but none as spectacular as those made in the last decade due to the development of novel techniques and the ability to sequence large stretches of DNA. In Chromatin Protocols, Second Edition, expert researchers delineate these cutting-edge techniques via step-by-step laboratory methods and protocols, which encompass a wide array of topics from the isolation of nucleosomes, assembly of nucleosomes and study of the basic chromatin structure to detailed analysis of histone modifications and chromatin function. Written in the highly successful Methods in Molecular Biology™ series style, chapters include brief introductions to the subjects, lists of the necessary materials and reagents, readily reproducible protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Chromatin Protocols, Second Edition is a valuable tool for scientists studying various aspects of chromatin function and an ideal guide to aid in the development of new techniques as well as new ideas in the field of chromatin biology.
Provides an easily accessible reference volume for analysis of chromatin structure and function incorporating the latest techniques Includes detailed, step by step guidelines for the analysis of chromatin structure and its role in transcription, replication and other biological processes Details elaborate protocols for the detection and characterization of histone modifications including acetylation, methylation and ubiquitination Describes protocols for techniques ranging from immunohistochemical detection of chromatin modifications to genome-wide analysis of chromatin associated proteins Acts as a comprehensive guide to the most up to date techniques dealing with all aspects of chromatin biology Presents experimental details in an easy to follow modular format typical of the MiMB series Includes supplementary material: sn.pub/extras
Autor*in
Srikumar P. Chellappan
Themen in »Chromatin Protocols«
Chromatin immunoprecipitation (ChIP) assays DNA DNA repair and replication Histones Nucleosomes Polymerase Chain Reaction Telomere hybridization molecular biology transcription gene expression
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From the reviews of the second edition:
“This book contains a collection of detailed and useful procedures concerning chromatin analysis. It includes methods for the isolation and assembly of nucleosomes, the analysis of basic chromatin structure, the covalent modification of histones and the analysis of chromatin function. … In summary, this is a very useful text, with lots of strengths … .” (Ray Waters, Human Genetics, Vol. 126, 2009)
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