Imaging Flow Cytometry
Methods and Protocols
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Beschreibung
This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols willbe a critical source for all laboratories seeking to implement IFC in their research studies.
Includes cutting-edge methods and protocols for imaging flow cytometry Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts
Autor*in
Natasha S. Barteneva
Themen in »Imaging Flow Cytometry«
Apoptosis Cell death analysis Drug screening IDEAS software IFC Immune response analysis Morphological analysis Nucleic acids Quantitative imaging flow cytometry
Stimmen zu »Imaging Flow Cytometry«
“The purpose is to present to the scientific community the compendium of recently developed imaging flow cytometry techniques and protocols. The authors are successful in achieving these worthy objectives. Intended for the scientific community, this book will be very useful for students, graduate students, and scientists interested in learning about imaging flow cytometry and its applications. … This book for the first time explores the techniques and protocols involving quantitative imaging flow cytometry in a fascinating manner.” (Omer Iqbal, Doody's Book Reviews, September, 2016)
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