Site-Specific Protein Labeling
Methods and Protocols
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Beschreibung
This detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information regarding the design and generation of the organic molecules used for the labeling step. Chapters present a comprehensive overview on the most relevant and established labeling methodologies, and help researchers to choose the most appropriate labeling method for their biological question. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Site-Specific Protein Labeling: Methods and Protocols is designed to help scientists from all fields to get a better understanding of labeling techniques.
This detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Chapters provide protocols for labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Site-Specific Protein Labeling: Methods and Protocols provides a comprehensive overview on the most relevant and established labeling methodologies, and helps researchers to choose the most appropriate labeling method for their biological question.
Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras
Autor*in
Arnaud Gautier
Themen in »Site-Specific Protein Labeling«
CLIP-tag technology FlAsH-tag SNAP-tag intracellular proteins labeled proteins membrane translocation protein translation site-specific protein labeling tag-based methods unnatural amino acids Protein Structure