Handbook of Biological Confocal Microscopy
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Beschreibung
This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.
Includes detailed descriptions and in-depth comparisons of parts of the microscope itself Covers dyes and techniques of 3D specimen prep, as well as digital data acquisition Addresses the fundamental limitations and practical complexities of quantitative confocal fluorescence imaging This is the revised and expanded Third Edition of a classic text Includes supplementary material: sn.pub/extras
Autor*in
James Pawley
Themen in »Handbook of Biological Confocal Microscopy«
Biological Confocal Microscopy Pawley chemistry databases electron microscopy fluorescence preparation