Handbook of Biological Confocal Microscopy
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Beschreibung
This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.
Autor*in
James Pawley
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Confocal Microscopy Pawley biological biology cell biology fluorescence information instruments laser lens optics photon quality system
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From the reviews of the third edition:
`Considerably augmented for [the] second edition...[contains] a vast amount of information....it will surely be much used.' Ultramicroscopy
"The audience for this book is primarily seniors and graduate students, core service operators, collaborating scientists, such as molecular biologists, physiologists, and clinicians who wish to complement and supplement their experience with sophisticated and quantitative microscopy. … The book is readable and instructive. … There are hundreds of diagrams, tables, and formulas, as well as hundreds of color images of the cells, tissues, organelles, and systems one can study with CFM. … The references are up to date, complete, and easy to find." (James B. Pawley, Veterinary Pathology, Vol. 45 (1), 2008)
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