Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration
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Beschreibung
A much-needed primer on the use of laser flow cytometry for stemcell analysis
Laser flow cytometry is a powerful tool for rapid analysis ofcells for marker expression, cell cycle position, proliferation,and apoptosis. However, no resources specifically address the useof this methodology for the study of stem cells; this is especiallyimportant as stem cell analysis involves specialized methods andstaining procedures based on specific characteristics such asmarker expression, cell size, drug transport, and efflux of thestem cells.
Now, this book reviews these procedures, discusses the sciencebehind them, and provides real-world examples to illustrate theusefulness of the methods. It brings together world-class expertsin pathology, biophysics, immunology, and stem cell research, whodraw upon their extensive experience with the methods and showexamples of good data to help guide researchers in the rightdirection.
Chapter coverage includes:
* Stem cell analysis and sorting using side population
* Flow cytometry in the study of proliferation and apoptosis
* Stem cell biology and application
* Identification and isolation of very small embryonic-like stemcells from murine and human specimens
* Hematopoietic stem cells--issues in enumeration
* Human embryonic stem cells: long-term culture andcardiovascular differentiation
* Limbal stem cells and corneal regeneration
* Flow cytometric sorting of spermatogonial stem cells
* Breast cancer stem cells
* Stem cell marker expression in cells from body cavityfluids
This book is an essential resource for all graduate students,practitioners in developing countries, libraries and bookrepositories of universities and research institutions, andindividual researchers. It is also of interest to laboratoriesengaged in stem cell research and use of stem cells for tissueregeneration, and to any organization dealing in stem cell andtissue regeneration research.
Autor*in
Awtar Krishan
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Biotechnologie i. d. Biowissenschaften Biotechnology Biowissenschaften Cell & Molecular Biology Cell Biology Durchflusszytometrie Gewebe (Physiol.) Life Sciences Stammzellenforschung Zell- u. Molekularbiologie Zellbiologie
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"Contributors from a wide range of medical and biological sciencesexplain methods and procedures of flow cytometry specific to itsuse in research on stem cells and related matters." (SciTechBook News, December 2010)
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