Functional Imaging by Controlled Nonlinear Optical Phenomena

Beschreibung

A comprehensive guide to theories, techniques, and technologies for the functional imaging of biological systems using nonlinear microscopy Nonlinear optical imaging using ultrafast laser pulses is a powerful microscopy technique that has become a valued research tool within the biological sciences. Allowing for very high-precision imaging and manipulation of cells, nonlinear optical imaging is revolutionizing our understanding of functional processes in living tissue, and the number of research and clinical applications for it continues to grow. Written by a team of distinguished experts from the University of Osaka, this is a comprehensive guide to state-of-the-art nonlinear microscopy of biological systems using ultrafast laser pulses. The authors review the evolution of nonlinear optical microscopy, from its origins in the 1990s to today's most advanced photo-controlled techniques, and explore an array of vital topics, including: * Conventional nonlinear optical microscopy * Ultrafast laser optics required for nonlinear optical microscopy * Ultrafast laser manipulation in biological and biomedical research * Functional imaging based on molecular control * The full range of current nonlinear optical microscopic techniques, from two-photon absorption microscopy to nonresonant four-wave mixing microscopy * An array of biological applications, including nanosurgery and transfection Providing balanced coverage of both theory and practice, Functional Imaging by Controlled Nonlinear Optical Phenomena is an indispensable primer for cell biologists, life scientists, and biomedical researchers, engineers, and technicians.
Ultrafast lasers allow high-precision imaging and manipulation for biological and medical applications. Nonlinear optical microscopy has provided researchers with unique possibilities of three-dimensional imaging of biological cells and tissues. Nonlinear optical imaging technique is a rapidly emerging research area with widespread fundamental research and clinical applications. Nonlinear optical imaging allows both structural and functional imaging with cellular level resolution imaging in biological systems. The introduction of endogenous or exogenous probes can selectively enhance contrast for molecular targets in a living cell as well as supply functional information on processes. With the aim to control nonlinear optical processes and to obtain functional images, nonlinear optical processes can be controlled by photo-controlled probes and/or parameters of ultrafast laser pulses, such as time, space, polarization, and phase. This book gives an overview of the nonlinear optical process by ultrafast laser pulses and explains how the basics of nonlinear optical microscopy led to the most advanced techniques of photo-controlled nonlinear optical microscopy.

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Kazuyoshi Itoh

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Bildgebende Verfahren i. d. Biomedizin Bildgebendes Verfahren Biomedical Engineering Biomedical Imaging Biomedizintechnik Electrical & Electronics Engineering Elektrotechnik u. Elektronik Nichtlineare Optik Optics & Photonics Optik u. Photonik Photonics & Lasers Photonik u. Laser Physics Physik

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"The authors have provided a remarkable reference on nonlinear optical and functional imaging, which includes powerful microscopy and spectroscopy techniques via ultrafast laser pulses . . . Researchers and graduate students interested in microscopy, spectroscopy and nonlinear optics would benefit from this book." (Optics & Photonics News, 4 April 2014)
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