Protein Purification
Principles, High Resolution Methods, and Applications
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Beschreibung
The authoritative guide on protein purification--nowcompletely updated and revised
Since the Second Edition of Protein Purification waspublished in 1998, the sequencing of the human genome and otherdevelopments in bioscience have dramatically changed the landscapeof protein research. This new edition addresses these developments,featuring a wealth of new topics and several chapters rewrittenfrom scratch.
Leading experts in the field cover all major biochemicalseparation methods for proteins in use today, providingprofessionals in biochemistry, organic chemistry, and analyticalchemistry with quick access to the latest techniques. Entirely newor thoroughly revised content includes:
* High-resolution reversed-phase liquid chromatography
* Electrophoresis in gels
* Conventional isoelectric focusing in gel slabs and capillariesand immobilized pH gradients
* Affinity ligands from chemical and biological combinatoriallibraries
* Membrane separations
* Refolding of inclusion body proteins from E. coli
* Purification of PEGylated proteins
* High throughput screening techniques in protein purification
* The history of protein chromatography
Autor*in
Jan-Christer Janson
Themen in »Protein Purification«
Analytical Chemistry Analytische Chemie Biochemie Biomolecules (DNA, RNA, Peptides, etc.) Biomoleküle (DNA, RNA, Peptide) Chemie Chemistry Protein Science Proteine