Prime Editing
Methods and Protocols
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Beschreibung
This book looks at the latest advancements in prime editing. The chapters in this book cover pegRNA design and assembly; optimizations for prime editing in mammals; plants and yeast; approaches for quantification of on-target and off-target events; and viral delivery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and practical, Prime Editing: Methods and Protocols is a valuable resource that provides researchers with detailed and practical methodology for implementing prime editing across a wide range of biological systems.
This book looks at the latest advancements in prime editing. The chapters in this book cover pegRNA design and assembly; optimizations for prime editing in mammals; plants and yeast; approaches for quantification of on-target and off-target events; and viral delivery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and practical, Prime Editing: Methods and Protocols is a valuable resource that provides researchers with detailed and practical methodology for implementing prime editing across a wide range of biological systems.
Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts
Autor*in
Peter M.J. Quinn
Themen in »Prime Editing«
CRISPR genome editing pegRNAs mRNA Production bacterial Prime Editing