RNA Remodeling Proteins
Methods and Protocols
Buch in deiner Nähe kaufen
Beschreibung
This third edition provides new and updated methods for the further study of RNA remodeling proteins. Chapters detail RNA remodeling proteins, AlphaFold to RNA-protein complexes, deep sequencing to probe transcriptome-wide remodeling events, helicase-dependent RNA structural changes, identifying helicase substrates, mapping transcriptional R-loops, leveraging techniques such as RNA-seq, DMS-seq, CLIP-seq, H-CRAC, Helicase-SELEX, proximity ligation, yeast three-hybrid assays, biochemical assays to measure RNA-protein affinity and helicase activities, structural and biophysical methods, an d functional characterization of RNA remodeling proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, RNA Remodeling Proteins: Methods and Protocols, Third Edition aims to ensure successful results in the further study of this vital field.
This third edition provides new and updated methods for the further study of RNA remodeling proteins. Chapters detail RNA remodeling proteins, AlphaFold to RNA-protein complexes, deep sequencing to probe transcriptome-wide remodeling events, helicase-dependent RNA structural changes, identifying helicase substrates, mapping transcriptional R-loops, leveraging techniques such as RNA-seq, DMS-seq, CLIP-seq, H-CRAC, Helicase-SELEX, proximity ligation, yeast three-hybrid assays, biochemical assays to measure RNA-protein affinity and helicase activities, structural and biophysical methods, an d functional characterization of RNA remodeling proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, RNA Remodeling Proteins: Methods and Protocols, Third Edition aims to ensure successful results in the further study of this vital field.
Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts
Autor*in
Marc Boudvillain
Themen in »RNA Remodeling Proteins«
Chemical footprinting Thermodynamic analysis Helicases eukaryotes prokaryotes