Botrytis cinerea
Methods and Protocols
Buch in deiner Nähe kaufen
Beschreibung
This volume provides comprehensive protocols on Botrytis cinerea and the main methods used to study this important fungal species. Chapters detail cover classical approaches, including isolation, cultural techniques, sclerotia induction, storage, microscopy and protein localization, inoculation assays on fruits and vegetables, antifungal susceptibility testing, protoplast production and transfection, RNA interference, and identification of mycoviral infections. Additionally, the volume presents state-of-the-art molecular and omics-based approaches, including high-quality DNA and RNA extraction, molecular marker development, genome assembly, quantitative gene expression analysis, and integrative transcriptomic, proteomic, and metabolomic analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Botrytis cinerea: Methods and Protocols aims to promote experimental rigor, facilitate cross-disciplinary collaboration, and stimulate innovative research directions in Botrytis biology.
This volume provides comprehensive protocols on Botrytis cinerea and the main methods used to study this important fungal species. Chapters detail cover classical approaches, including isolation, cultural techniques, sclerotia induction, storage, microscopy and protein localization, inoculation assays on fruits and vegetables, antifungal susceptibility testing, protoplast production and transfection, RNA interference, and identification of mycoviral infections. Additionally, the volume presents state-of-the-art molecular and omics-based approaches, including high-quality DNA and RNA extraction, molecular marker development, genome assembly, quantitative gene expression analysis, and integrative transcriptomic, proteomic, and metabolomic analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Botrytis cinerea: Methods and Protocols aims to promote experimental rigor, facilitate cross-disciplinary collaboration, and stimulate innovative research directions in Botrytis biology.
Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes from leading experts
Autor*in
Luca Nerva
Themen in »Botrytis cinerea«
DNA isolation transcriptome analysis Plant–Fungal Pathogen Interactions Morphological identification RNAi