Synthetic Gene Circuits
Methods and Protocols
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Beschreibung
This detailed volume presents experimental and computational methods for constructing and interrogating synthetic gene circuits across multiple organizational scales. Beginning with a section on genetic tools, platforms, and experimental frameworks that enable sophisticated circuit construction, the book continues by covering methods for regulating circuit behavior and mitigating variability, as well as frameworks for understanding time-dependent circuit behavior in growing cells and populations. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical, Synthetic Gene Circuits: Methods and Protocols aims to support a synthetic biology practice that is predictive by design, robust by control, and interpretable through dynamics.
This detailed volume presents experimental and computational methods for constructing and interrogating synthetic gene circuits across multiple organizational scales. Beginning with a section on genetic tools, platforms, and experimental frameworks that enable sophisticated circuit construction, the book continues by covering methods for regulating circuit behavior and mitigating variability, as well as frameworks for understanding time-dependent circuit behavior in growing cells and populations. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical, Synthetic Gene Circuits: Methods and Protocols aims to support a synthetic biology practice that is predictive by design, robust by control, and interpretable through dynamics.
Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts
Autor*in
Xiao-Jun Tian
Themen in »Synthetic Gene Circuits«
Synthetic biology Circuit design Genetic design Synthetic biological systems Circuit behavior Cell-free systems Living systems CRISPR Machine learning