SMC Proteins
Methods and Protocols
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Beschreibung
The aim of this volume is to provide a wide range of experimental methods for analyzing molecular functions of SMC complexes, such as microscopic imaging, protein localization, chromosome conformation analyses by deep DNA sequencing and biochemical reconstitution with purified proteins or cell lysate. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, SMC Proteins: Methods and Protocols aims to be comprehensive guide for researchers in the field.
The aim of this volume is to provide a wide range of experimental methods for analyzing molecular functions of SMC complexes, such as microscopic imaging, protein localization, chromosome conformation analyses by deep DNA sequencing and biochemical reconstitution with purified proteins or cell lysate. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, SMC Proteins: Methods and Protocols aims to be comprehensive guide for researchers in the field.
Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts
Autor*in
Yasuto Murayama
Themen in »SMC Proteins«
DNA loop extrusion bacterial SMC proteins subunit-subunit interactions dTag degradation SUMOylation