Duchenne Muscular Dystrophy
Methods and Protocols
Buch in deiner Nähe kaufen
Beschreibung
This volume discusses protocols for key laboratory techniques used to study Duchenne and Becker muscular dystrophies. The chapters in this book cover a wide array of topics such as biochemical assays, quantification of dystrophin and related proteins, and measurements of muscle physiology. The chapters also cover methods of DMD sequence analysis; processing and isolation of cells from muscle tissue; culture and study of patient-derived cells; and work with animal models of DMD. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Duchenne Muscular Dystrophy: Methods and Protocols is a valuable resource that will contribute to the standardization of practices in the study of dystrophin and dystrophin-related disease.
This volume discusses protocols for key laboratory techniques used to study Duchenne and Becker muscular dystrophies. The chapters in this book cover a wide array of topics such as biochemical assays, quantification of dystrophin and related proteins, and measurements of muscle physiology. The chapters also cover methods of DMD sequence analysis; processing and isolation of cells from muscle tissue; culture and study of patient-derived cells; and work with animal models of DMD. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Duchenne Muscular Dystrophy: Methods and Protocols is a valuable resource that will contribute to the standardization of practices in the study of dystrophin and dystrophin-related disease.
Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts
Autor*in
Kevin Flanigan
Themen in »Duchenne Muscular Dystrophy«
next generation sequencing immunohistochemical analysis mass spectrometry dystrophin expression capillary immunoassay