Schizosaccharomyces pombe
Methods and Protocols
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Beschreibung
This detailed volume explores techniques utilizing the fission yeast Schizosaccharomyces pombe, a powerful tractable model system for eukaryotic biology. The book begins with a section on microscopy and imaging, and it continues with chapters covering genome and plasmid editing, including the use of CRISPR/Cas9 technologies, as well as the analysis of DNA damage, repair, and recombination, and more. The volume also includes chapters with a focus on computational methods specific to the approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Schizosaccharomyces pombe: Methods and Protocols serves as the ideal guide for researchers in the fission yeast community working with emerging technologies.
This detailed volume explores techniques utilizing the fission yeast Schizosaccharomyces pombe, a powerful tractable model system for eukaryotic biology. The book begins with a section on microscopy and imaging, and it continues with chapters covering genome and plasmid editing, including the use of CRISPR/Cas9 technologies, as well as the analysis of DNA damage, repair, and recombination, and more. The volume also includes chapters with a focus on computational methods specific to the approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Schizosaccharomyces pombe: Methods and Protocols serves as the ideal guide for researchers in the fission yeast community working with emerging technologies.
Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts
Autor*in
Ruben C. Petreaca
Themen in »Schizosaccharomyces pombe«
Fission yeast Eukaryotic biology CRISPR/Cas9 Microscopy analysis Genome and plasmid editing DNA damage Engineered mutations