Antiviral Innate Immunity
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Beschreibung
This detailed volume explores a variety of techniques for studying antiviral innate immunity and the type I interferon (IFN-I) signaling pathway. Beginning with guidelines to identify novel proteins from the host or viruses that regulate the antiviral IFN-I signaling pathway, the book continues with common and cutting-edge approaches for identifying novel regulatory factors, posttranslational modifications, as well as click chemistry to detect protein modifications. Other chapters delve into topics such as cytosolic RNA and DNA sensors, magnetic beads, cryo-electron microscopy, zebrafish, humanized mice, and organoids as model organisms to study host-virus interactions, and epidemiological approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Antiviral Innate Immunity serves as an ideal guide for researchers working in this vital area of the virology and innate immunity fields.
This detailed volume explores a variety of techniques for studying antiviral innate immunity and the type I interferon (IFN-I) signaling pathway. Beginning with guidelines to identify novel proteins from the host or viruses that regulate the antiviral IFN-I signaling pathway, the book continues with common and cutting-edge approaches for identifying novel regulatory factors, posttranslational modifications, as well as click chemistry to detect protein modifications. Other chapters delve into topics such as cytosolic RNA and DNA sensors, magnetic beads, cryo-electron microscopy, zebrafish, humanized mice, and organoids as model organisms to study host-virus interactions, and epidemiological approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Antiviral Innate Immunity serves as an ideal guide for researchers working in this vital area of the virology and innate immunity fields.
Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts
Autor*in
Chunfu Zheng
Themen in »Antiviral Innate Immunity«
Type I interferon (IFN-I) signaling pathway Viral infections Novel regulatory factors CRISPR-mediated library screening Signal transduction