Patient-Derived Xenografts
Methods and Protocols
Buch in deiner Nähe kaufen
Beschreibung
This detailed volume explores the dynamic landscape of patient-derived xenograft (PDX) generation and applications. After a few chapters examining the evolution of PDXs, tracing their roots and contextualizing their significance in the broader narrative of cancer research, the book continues with methodologies for generating PDXs from diverse clinical tissue samples as well as for utilizing PDXs in various specific cancer types. From the development of humanized PDX models to the exploration of protein expression through advanced techniques including multiplex immunohistochemistry, Western blotting, and immunoprecipitation, the chapters offer a comprehensive toolkit for researchers and clinicians alike. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Patient-Derived Xenografts: Methods and Protocols serves as an ideal interdisciplinary guide for understanding the intricate processes that underscore translational cancer research.
This detailed volume explores the dynamic landscape of patient-derived xenograft (PDX) generation and applications. After a few chapters examining the evolution of PDXs, tracing their roots and contextualizing their significance in the broader narrative of cancer research, the book continues with methodologies for generating PDXs from diverse clinical tissue samples as well as for utilizing PDXs in various specific cancer types. From the development of humanized PDX models to the exploration of protein expression through advanced techniques including multiplex immunohistochemistry, Western blotting, and immunoprecipitation, the chapters offer a comprehensive toolkit for researchers and clinicians alike. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Patient-Derived Xenografts: Methods and Protocols serves as an ideal interdisciplinary guide for understanding the intricate processes that underscore translational cancer research.
Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts
Autor*in
Mohamed I. Saad
Themen in »Patient-Derived Xenografts«
PDXs Cancer therapeutics Translational cancer research Clinical tissue samples Cancer types Protein expression Predictive biomarkers Drug development