Germ Cell Development
Methods and Protocols
Buch in deiner Nähe kaufen
Beschreibung
This detailed volume explores techniques for the isolation, purification, and establishment of in vitro germ cell (GS) systems at different stages of development in a variety of organisms, including chickens, mice, rats, and humans. The book additionally describes cutting-edge analytic and informatic tools to study germ cell development at the single cell level and meiotic recombination. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Germ Cell Development: Methods and Protocols provides new insight into the understanding of basic biological aspects of germ cell biology, as well as the opportunity for in vitro manipulation of germ cells for toxicology studies and for genetic intervention.
This detailed volume explores techniques for the isolation, purification, and establishment of in vitro germ cell (GS) systems at different stages of development in a variety of organisms, including chickens, mice, rats, and humans. The book additionally describes cutting-edge analytic and informatic tools to study germ cell development at the single cell level and meiotic recombination. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Germ Cell Development: Methods and Protocols provides new insight into the understanding of basic biological aspects of germ cell biology, as well as the opportunity for in vitro manipulation of germ cells for toxicology studies and for genetic intervention.
Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts
Autor*in
Marco Barchi
Themen in »Germ Cell Development«
In vitro GC systems Primordial germ cells (PGCs) Animal models Cytological methods Genetic information transfer