Dynein
Methods and Protocols
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Beschreibung
This volume provides a collection of protocols from leading researchers in the dynein field. The enclosed chapters guide readers through a broad range of powerful techniques that can be used to understand the complexities of dynein activity and regulation. These include how to perform live-cell microscopy on various fungi and metazoan cells, how to purify, assemble, and measure the force generation and motility characteristics of various dynein assemblies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Dynein: Methods and Protocols is a valuable resource for both novice and expert scientists in this developing field.
This volume provides a collection of protocols from leading researchers in the dynein field. The enclosed chapters guide readers through a broad range of powerful techniques that can be used to understand the complexities of dynein activity and regulation. These include how to perform live-cell microscopy on various fungi and metazoan cells, how to purify, assemble, and measure the force generation and motility characteristics of various dynein assemblies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Dynein: Methods and Protocols is a valuable resource for both novice and expert scientists in this developing field.
Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts
Autor*in
Steven M. Markus
Themen in »Dynein«
organelles DNA origami complex molecular machines molecular motor interkinetic nuclear migration spindle assembly