Ayyar Affinity Chromatography

Affinity Chromatography

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Methods and Protocols

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Beschreibung

This volume covers various aspects of affinity chromatography along with examples of its applications. Chapters guide readers through methodologies to purify a diverse array of molecular targets such as antibodies, extracellular vesicles, recombinant proteins, biomarkers, metabolites, plant organelles, nucleic acids, ligand identification and protocols on building affinity matrix. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 Authoritative and cutting-edge, Affinity Chromatography: Methods and Protocols aims to be an invaluable resource to anyone employing affinity chromatography-based methodologies.


This volume covers various aspects of affinity chromatography along with examples of its applications. Chapters guide readers through methodologies to purify a diverse array of molecular targets such as antibodies, extracellular vesicles, recombinant proteins, biomarkers, metabolites, plant organelles, nucleic acids, ligand identification and protocols on building affinity matrix. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 Authoritative and cutting-edge, Affinity Chromatography: Methods and Protocols aims to be an invaluable resource to anyone employing affinity chromatography-based methodologies.


Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Autor*in

B. Vijayalakshmi Ayyar

Themen in »Affinity Chromatography«

Fab Fragments E. coli protein purification antibody methylation

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Details

ISBN: 9781071621769
Verlag: Springer US
Erscheinung: 18.05.2022

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