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Fluorescent Microscopy

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Beschreibung

This volume provides both experienced and new microscopists with methods and protocols to perform fluorescence microscopy-based experiments. The book is divided into four parts detailing basic fluorescent microscopy, quantitative methods, imaging living animals, human tissue samples, approaches for imaging at a near-molecular level, and approaches to image analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Fluorescent Microscopy aims to be a useful practical guide to researches to help further their study in this field. 


This volume provides both experienced and new microscopists with methods and protocols to perform fluorescence microscopy-based experiments. The book is divided into four parts detailing basic fluorescent microscopy, quantitative methods, imaging living animals, human tissue samples, approaches for imaging at a near-molecular level, and approaches to image analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Fluorescent Microscopy aims to be a useful practical guide to researches to help further their study in this field. 


Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Autor*in

Bryan Heit

Themen in »Fluorescent Microscopy«

Live-Cell Imaging Foster Resonant Energy Transfer (FRET) Fluorescence Lifetime Imaging Microscopy (FLIM). Bimolecular Fluorescence Complementation (BiFC). Total Internal Reflection Microscopy (TIRF).

Stimmen zu »Fluorescent Microscopy«

Details

ISBN: 9781071620533
Verlag: Springer US
Erscheinung: 28.02.2023

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