Principles and Practices of DNA Analysis: A Laboratory Manual for Forensic DNA Typing
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Beschreibung
The book presents hands-on protocols for conventional and advanced forensic DNA fingerprinting experiments. It includes manual, semi-automatic, and advanced automatic techniques for DNA extraction from different biological samples.
It also discusses various qualitative and quantitative approaches for the assessment of extracted forensic DNA. It contains protocols for the amplification of short tandem repeat markers (STRs) for the amplification-based target enrichment of the forensic samples.
Further, it examines genotyping of the STR loci through capillary electrophoresis and includes real-world case studies where forensic DNA analysis has been used in the criminal and civil disputes. The book concludes by presenting technological developments in the field of DNA forensic analysis.
Suitable for beginners, it is a key reference resource on a wide variety of DNA profiling techniques and applications.
The book presents hands-on protocols for conventional and advanced forensic DNA fingerprinting experiments. It includes manual, semi-automatic, and advanced automatic techniques for DNA extraction from different biological samples.
It also discusses various qualitative and quantitative approaches for the assessment of extracted forensic DNA. It contains protocols for the amplification of short tandem repeat markers (STRs) for the amplification-based target enrichment of the forensic samples.
Further, it examines genotyping of the STR loci through capillary electrophoresis and includes real-world case studies where forensic DNA analysis has been used in the criminal and civil disputes. The book concludes by presenting technological developments in the field of DNA forensic analysis.
Suitable for beginners, it is a key reference resource on a wide variety of DNA profiling techniques and applications.
Includes the basic principles of DNA fingerprinting Provides step-by-step protocols for DNA extraction, STR amplification, genotyping, and statistical analysis Examines criminal and civil cases where DNA profiling has been used as forensic evidence Outlines recent technological advances in DNA fingerprinting analysis
Autor*in
Hirak Ranjan Dash
Themen in »Principles and Practices of DNA Analysis: A Laboratory Manual for Forensic DNA Typing«
DNA Fingerprinting Protocols STR markers DNA Genotyping NGS Mitochondrial DNA analysis