RNA'Protein Interaction Protocols

Beschreibung

In RNA-Protein Interaction Protocols, highly skilled experimentalists provide a comprehensive series of commonly used, as well as specialized, techniques for analyzing how proteins and RNA interact. Richly detailed and readily reproducible, these methods enable researchers to analyze the structural details of an RNA-protein interaction, to determine in detail what parts of the protein and RNA are in close contact, and to isolate RNP complexes from cells. There are also in vitro and in vivo methods and protocols for assaying the effects of proteins and RNP complexes on mRNA metabolism. The methods assume only a knowledge of basic molecular biology, biochemistry, and cell culture, and can be readily adapted to other systems.
Providing an excellent standard resource for today's bench scientists studying many types of RNA-protein interactions, RNA-Protein Interaction Protocols offers a wide-ranging collection of up-to-date experimental methods that distill a great deal of wisdom and experience into productive, reproducible, step-by-step techniques for all investigators in the field.

The molecular characterization of RNA and its interactions with proteins is an important and exciting area of current research. Organisms utilize a variety of RNA–protein interactions to regulate the expression of their genes. This is particularly true for eukaryotes, since newly synthesized messenger RNA must be extensively modified and transported to the cytoplasm before it can be used for protein synthesis. The realization that posttranscriptional processes are critical components of gene regulation has sparked an explosion of interest in both stable ribonucleoprotein (RNP) complexes and transient RNA–protein interactions. RNA is conformationally flexible and can adopt complex structures that provide diverse surfaces for interactions with proteins. The fact that short RNA molecules (aptamers; see Chapter 16) can be selected to bind many different types of molecules is evidence of the structural variability of RNA. RNA molecules are rarely entirely single- or double-stranded, but usually contain multiple short duplexes interrupted by single-stranded loops and bulges; in some RNAs, such as tRNAs, the short duplexes stack on each other. Further variability is generated by the presence of non-Watson-Crick base pairs, modified nucleotides, and more complex structures, such as pseudoknots and triple-strand interactions.

Autor*in

Susan R. Haynes

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"This manual is recommended to investigators with experience in molecular biological research, wishing to become introduced into the field of RNA-protein interactions. This book will also be a valuable source of reference for scientists needing general information about the theoretical basis and current methodological approaches to investigating ribonucleoprotein complexes."-International Journal of Medical Microbiology

"The coverage of the book is broad and reasonably comprehensive, with chapters arranged in a coherent sequence....Many of the authors are well-known, leading researchers in their field, and describe key contemporary techniques. In these respects the coverage is authoritative and likely to remain relevant for some years, before being overtaken by new technologies and methods...represents value for money."-Cell Biology International

"This is a book which any scientist involved in any kind of cellular and molecular biology will find profitable for his experimental investigations since RNA and proteins are unavoidable matters to solve any problem in Life Sciences. Highly skilled experimentalists provide a comprehensive series of commonly used, as well as specialized techniques for analyzing how proteins and RNA interact. Richly detailed and readily reproducible, these methods enable researchers to analyze the structural details of an RNA-protein interaction, to determine in detail what parts of the protein and RNA are in close contact, and to isolate RNP complexes from cells." - Cellular and Molecular Biology
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