Most research in the life sciences involves a core set ofmolecular-based equipment and methods, for which there is noshortage of step-by-step protocols. Nonetheless, there remains anexceedingly high number of inquiries placed to commercial technicalsupport groups, especially regarding problems.
Molecular Biology Problem Solver: A LaboratoryGuide asks the reader to consider crucial questions, suchas:
* Have you selected the most appropriate research strategy?
* Have you identified the issues critical to your successfulapplication of a technique?
* Are you familiar with the limitations of a giventechnique?
* When should common procedural rules of thumb not beapplied?
* What strategies could you apply to resolve a problem?
A unique question-based format reviews common assumptions andlaboratory practices, with the aim of offering a firm understandingof how techniques and procedures work, as well as how to avoidproblems. Some major issues explored by the book's expertcontributors include:
* Working safely with biological samples and radioactivematerials
* DNA and RNA purification
* PCR
* Protein and nucleid acid hybridization
* Prokaryotic and eukaryotic expression systems
* Properly using and maintaining laboratory equipment
Alan S. Gerstein
Biologie / Grundlagen Biowissenschaften Cell & Molecular Biology Life Sciences Zell- u. Molekularbiologie
"It will strengthen your ability to prevent, identify and solve problems in the lab..." (Medical Laboratory World, October 2001)
()