Protein Sequencing and Identification Using Tandem Mass Spectrometry

Beschreibung

How to design, execute, and interpret experiments for protein sequencing using mass spectrometry The rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing. This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation. Michael Kinter and Nicholas Sherman present their own high-quality, laboratory-tested protocols for the analysis of a wide variety of samples, demonstrating how to carry out specific experiments and obtain fast, reliable results with a 99% success rate. Readers will get sufficient experimental detail to apply in their own laboratories, learn about the proper selection and operation of instruments, and gain essential insight into the fundamental principles of mass spectrometry and protein sequencing. Coverage includes: * Peptide fragmentation and interpretation of product ion spectra * Basic polyacrylamide gel electrophoresis * Preparation of protein digests for sequencing experiments * Mass spectrometric analysis using capillary liquid chromatography * Techniques for protein identification by database searches * Characterization of modified peptides using tandem mass spectrometry And much more
Die rasche Entwicklung der Proteinsequenzierung innerhalb der letzten zwei bis drei Jahre ist vor allem drei Aspekten zu verdanken: der Anwendung neuartiger Verfahren zum Proteinabbau, der Erweiterung der DNA-Sequenz-Datenbanken (z. B. vom National Center for Biotechnology Information) und der Verfügbarkeit von Computerprogrammen zum effizienten Durchsuchen dieser Datenbestände. Dieser hochaktuelle erläutert den gesamten Prozeß der Proteinsequenzierung durch Massenspektrometrie - von der Probenvorbereitung und Analyse bis zur Interpretation. Mit vielen praxisnahen Tipps! (11/00)

Autor*in

Michael Kinter

Themen in »Protein Sequencing and Identification Using Tandem Mass Spectrometry«

Biotechnologie i. d. Chemie Biotechnology Chemie Chemistry Mass Spectrometry Massenspektrometrie Proteine

Stimmen zu »Protein Sequencing and Identification Using Tandem Mass Spectrometry«

"[the authors] present.laboratory-tested protocols for the analysis of a wide variety of samples, providing sufficient detail for duplication of their methods." (SciTech Book News Vol. 25, No. 2 June 2001) ".an informative, well-written and well-illustrated book with a large number of useful references." (Spectroscopyeurope.com)
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"[the authors] present their own laboratory-tested protocols for the analysis of a wide variety of samples, providing sufficient detail for duplication of their methods." (SciTech Book News Vol. 25, No. 2 June 2001) ".an informative, well-written and well-illustrated book with a large number of useful references. It should prove invaluable to anyone working in the field of protein sequencing." (Spectroscopyeurope.com)
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