Assay Development
Fundamentals and Practices
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Beschreibung
Essential principles and practice of assay development
The first comprehensive, integrated treatment of the subject,Assay Development: Fundamentals and Practices covers the essentialsand techniques involved in carrying out an assay project in eithera biotechnology/drug discovery setting or a platform setting.
Rather than attempting comprehensive coverage of all assaydevelopment technologies, the book introduces the most widely usedassay development technologies and illustrates the art of assaydevelopment through a few commonly encountered biological targetsin assay development (e.g., proteases, kinases, ion channels, and Gprotein-coupled receptors). Just enough biological background forthese biological targets is provided so that the reader can followthe logics of assay development. Chapters discuss:
* The basics of assay development, including foundational conceptsand applications
* Commonly used instrumental methods for both biochemical assaysand cell-based assays
* Assay strategies for protein binding and enzymatic activity
* Cell-based assays
* High-throughput screening
An in-depth study of the now popular Caliper's off-chip kinaseassay provides an instructive, real-world example of the assaydevelopment process.
Autor*in
Ge Wu
Themen in »Assay Development«
Analytical Chemistry Analytische Chemie Biowissenschaften Cell & Molecular Biology Chemie Chemistry Life Sciences Pharmaceutical & Medicinal Chemistry Pharmazeutische u. Medizinische Chemie Test Zell- u. Molekularbiologie
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"Although it is not an easy task to satisfy the demands of all scientists, the book Assay Development is an important treaise for scientits of modern biology". (ChemBioChem, 7 March 2011)
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