Handbook of Single-Molecule Biophysics
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Beschreibung
The last decade has seen the development of a number of novel biophysical methods that allow the manipulation and study of individual biomolecules. The ability to monitor biological processes at this fundamental level of sensitivity has given rise to an improved understanding of the underlying molecular mechanisms. Through the removal of ensemble averaging, distributions and fluctuations of molecular properties can be characterized, transient intermediates identified, and catalytic mechanisms elucidated. By applying forces on biomolecules while monitoring their activity, important information can be obtained on how proteins couple function to structure. The Handbook of Single-Molecule Biophysics provides an introduction to these techniques and presents an extensive discussion of the new biological insights obtained from them. Coverage includes:
- Experimental techniques to monitor and manipulate individual biomolecules
- The use of single-molecule techniques in super-resolution and functional imaging
- Single-molecule studies of physical properties of biomolecules
- Single-molecule enzymology and biochemistry
- Single molecules in the membrane
- Single-molecule techniques in living cells
- Integration of single-molecule biophysics and nanoscience
During the last decade, a number of novel biophysical methods have been developed that allow the manipulation and study of individual biomolecules. The ability to monitor biological processes at the fundamental level of sensitivity, that of a single molecule, has given rise to an improved understanding of the underlying molecular mechanisms. Through the removal of ensemble averaging, distributions and fluctuations of molecular properties can be characterized, transient intermediates identified, and catalytic mechanisms elucidated. By applying forces on biomolecules while monitoring their activity, important information can be obtained on how proteins couple function to structure. The Handbook of Single-Molecule Biophysics provides an introduction to these techniques and presents an extensive discussion of the new biological insights obtained from them.
Editorial Advisory Board: Daniel Müller, Cheng Zhu, Claus Seidel, Xiaowei Zhuang, Thomas Schmidt, Nynke Dekker.
Describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping Addresses the use of single-molecule techniques in super-resolution and functional imaging Includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells Integrates single-molecule biophysics and nanoscience
Autor*in
Peter Hinterdorfer
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Biomembran Biophysics FRET cells fluorescence imaging microscopy molecule proteins
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From the reviews:
“The Handbook of Single-Molecule Biophysics, edited by Hinterdorfer (Johannes Kepler Univ. of Linz, Austria) and van Oijen (Harvard Medical School), contains 21 chapters that address various aspects of techniques used to investigate single-molecule biophysics. The chapters, many authored by leading experts in their respective fields, cover specialized biophysical techniques, their applications, background into the technical design, and practical aspects of implementing these approaches. … Summing Up: Recommended. Graduate students, researchers, faculty, and professionals.” (C. A. Reinhart-King, Choice, Vol. 47 (10), June, 2010)
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